Effects of direct-fed microbial supplementation on performance and immune parameters of lactating dairy cows

作者：Adeoye Oyebade, Sang‐Cheol Lee, Halima Sultana, K.G. Arriola, Emmanuel Duvalsaint, C. Nino De Guzman, Ignacio Fernandez Marenchino, L. Marroquin Pacheco, F.X. Amaro, Lucas Ghedin Ghizzi, Lin Mu, Hui Guan, Kleves V Almeida, B. Rajo Andrade, Jie Zhao, Pengfei Tian, Chih‐Chien Cheng, Yun Jiang, John P. Driver, O.C.M. Queiroz, L.F. Ferraretto, Ibukun M Ogunade, A.T. Adesogan, D. Vyas · 发表于：Journal of Dairy Science · 年份：2023 · DOI：10.3168/jds.2022-22898 · 被引用次数：22 · 研究领域：Ruminant Nutrition and Digestive Physiology、Reproductive Physiology in Livestock、Animal health and immunology

We evaluated the effects of supplementing bacterial direct-fed microbial (DFM) on performance, apparent total-tract digestibility, rumen fermentation, and immune parameters of lactating dairy cows. One-hundred and 14 multiparous Holstein cows (41 ± 7 DIM) were used in a randomized complete block design with an experiment comprising 14-d covariate (pre-experimental sample and data collection) and 91-d of experimental period. Cows were blocked based on energy-corrected milk yield during the covariate period and the following treatments were randomly assigned within each block: 1) Control ( CON ): Corn silage-based total mixed ration (TMR) without DFM; 2) PRO-A : Basal diet top-dressed with a mixture of Lactobacillus animalis and Propionibacterium freudenreichii at 3 × 10 9 cfu/day; and 3) PRO-B : Basal diet top-dressed with a mixture of L. animalis , P. freudenreichii, Bacillus subtilis and B. licheniformis at 11.8 × 10 9 cfu/day. Milk yield, DMI, and BW were measured daily while milk samples for component analysis were taken on 2 consecutive days of each week of data collection. Feces, urine, rumen, and blood samples were taken during the covariate period, wk 4, 7, 10, and 13 for estimation of digestibility, N-partitioning, rumen fermentation, plasma nutrient status and immune parameters. Treatments had no effect on DMI and milk yield. Fat-corrected milk (3.5% FCM) and milk fat yield were improved with PRO-B, while milk fat percent and feed efficiency (ECM/DMI) tended to incre...