Wounding induces a peroxisomal H 2 O 2 decrease via glycolate oxidase‐catalase switch dependent on glutamate receptor‐like channel‐supported Ca 2+ signaling in plants

作者：Xiangyang Li, Linru Chen, Xiaoyue Zeng, Kaixin Wu, Jiayu Huang, Mengmeng Liao, Yue Xi, Guohui Zhu, Xiuying Zeng, Xuewen Hou, Zhisheng Zhang, Xinxiang Peng · 发表于：The Plant Journal · 年份：2023 · DOI：10.1111/tpj.16427 · 被引用次数：14 · 研究领域：Plant and Biological Electrophysiology Studies、Plant Stress Responses and Tolerance、GABA and Rice Research

SUMMARY Sensing of environmental challenges, such as mechanical injury, by a single plant tissue results in the activation of systemic signaling, which attunes the plant's physiology and morphology for better survival and reproduction. As key signals, both calcium ions (Ca 2+ ) and hydrogen peroxide (H 2 O 2 ) interplay with each other to mediate plant systemic signaling. However, the mechanisms underlying Ca 2+ ‐H 2 O 2 crosstalk are not fully revealed. Our previous study showed that the interaction between glycolate oxidase and catalase, key enzymes of photorespiration, serves as a molecular switch (GC switch) to dynamically modulate photorespiratory H 2 O 2 fluctuations via metabolic channeling. In this study, we further demonstrate that local wounding induces a rapid shift of the GC switch to a more interactive state in systemic leaves, resulting in a sharp decrease in peroxisomal H 2 O 2 levels, in contrast to a simultaneous outburst of the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase‐derived apoplastic H 2 O 2 . Moreover, the systemic response of the two processes depends on the transmission of Ca 2+ signaling, mediated by glutamate‐receptor‐like Ca 2+ channels 3.3 and 3.6. Mechanistically, by direct binding and/or indirect mediation by some potential biochemical sensors, peroxisomal Ca 2+ regulates the GC switch states in situ , leading to changes in H 2 O 2 levels. Our findings provide new insights into the functions of photorespiratory H 2 O 2 in plant...