Self-Renewable Tag for Photostable Fluorescence Imaging of Proteins

作者：Zhichao Du, Wenjing Wang, Siyuan Luo, Lingjie Zhang, Shuowei Yuan, Yongzhen Hei, Zhangbin Bao, Chunlai Chen, Yi Lin, Ling Chu · 发表于：Journal of the American Chemical Society · 年份：2023 · DOI：10.1021/jacs.3c06102 · 被引用次数：22 · 研究领域：Click Chemistry and Applications、Advanced biosensing and bioanalysis techniques、Advanced Biosensing Techniques and Applications

We report the development of a self-renewable tag (srTAG) for protein fluorescence imaging. srTAG leverages the "on-protein" fluorophore equilibrium between the fluorescent zwitterion and non-fluorescent spirocyclic form and the reversible fluorescence labeling to enable self-recovery of fluorescence after photobleaching. This small-sized srTAG allows 2-6 times longer imaging duration compared to other commonly used self-labeling tags and is compatible with fluorophores with different spectral properties. This study provides a new strategy for fine tuning of self-labeling tags.