Inducing the “re-development state” of periodontal ligament cells via tuning macrophage mediated immune microenvironment

作者：Guanqi Liu, Linjun Zhang, Xuan Zhou, Junlong Xue, Ruidi Xia, Xuejing Gan, Chunxiao Lv, Yanshu Zhang, Xueli Mao, Xiaoxing Kou, Songtao Shi, Zetao Chen · 发表于：Journal of Advanced Research · 年份：2023 · DOI：10.1016/j.jare.2023.08.009 · 被引用次数：38 · 研究领域：Periodontal Regeneration and Treatments、Dental Trauma and Treatments、Oral microbiology and periodontitis research

INTRODUCTION: Periodontal regeneration, specifically the restoration of the cementum-periodontal ligament (PDL)-alveolar bone complex, remains a formidable challenge in the field of regenerative dentistry. In light of periodontal development, harnessing the multi-tissue developmental capabilities of periodontal ligament cells (PDLCs) and reinitiating the periodontal developmental process hold great promise as an effective strategy to foster the regeneration of the periodontal complex. OBJECTIVES: This study aims to delve into the potential effects of the macrophage-mediated immune microenvironment on the "developmental engineering" regeneration strategy and its underlying molecular mechanisms. METHODS: In this study, we conducted a comprehensive examination of the periodontium developmental process in the rat mandibular first molar using histological staining. Through the induction of diverse immune microenvironments in macrophages, we evaluated their potential effects on periodontal re-development events using a cytokine array. Additionally, we investigated PDLC-mediated periodontal re-development events under these distinct immune microenvironments through transcriptome sequencing and relevant functional assays. Furthermore, the underlying molecular mechanism was also performed. RESULTS: The activation of development-related functions in PDLCs proved challenging due to their declined activity. However, our findings suggest that modulating the macrophage immune response can ...