Endophytic Beauveria bassiana promotes plant biomass growth and suppresses pathogen damage by directional recruitment

作者：Li Sui, Yang Lu, Linyan Zhou, Nannan Li, Qiyun Li, Zhengkun Zhang · 发表于：Frontiers in Microbiology · 年份：2023 · DOI：10.3389/fmicb.2023.1227269 · 被引用次数：54 · 研究领域：Entomopathogenic Microorganisms in Pest Control、Plant and fungal interactions、Plant-Microbe Interactions and Immunity

Introduction Entomopathogenic fungi (EPF) can colonize and establish symbiotic relationships with plants as endophytes. Recently, EPF have been reported to suppress plant pathogens and induce plant resistance to diseases. However, the potential mechanisms via which EPF as endophytes control major plant diseases in situ remain largely unknown. Methods Pot and field experiments were conducted to investigate the mechanisms via which an EPF, Beauveria bassiana , colonizes tomato, under Botrytis cinerea infection stress. B. bassiana blastospores were inoculated into tomato plants by root irrigation. Tomato resistance to tomato gray mold caused by B. cinerea was evaluated by artificial inoculation, and B. bassiana colonization in plants and rhizosphere soil under B. cinerea infection stress was evaluated by colony counting and quantitative PCR. Furthermore, the expression levels of three disease resistance-related genes ( OXO , CHI , and atpA ) in tomato leaves were determined to explore the effect of B. bassiana colonization on plant disease resistance performance in pot experiments. Results B. bassiana colonization could improve resistance of tomato plants to gray mold caused by B. cinerea . The incidence rate, lesion diameter, and disease index of gray mold decreased in both the pot and field experiments following B. bassiana colonization. B. bassiana was more likely to accumulate in the pathogen infected leaves, while decreasing in the rhizosphere soil, and induced the expressi...