Traditional Chinese Medicine formula Bu-Shen-Huo-Xue-Fang (BSHXF) protects nucleus pulposus cells against the inflammatory and oxidative stress-induced degenerative changes

作者：Linghui Li, Xu Wei, Kaiming Li, Hao Gong, Liguo Zhu, Shaofeng Yang, Shang-Quan Wang, Jinyu Gu, Ming Chen, Xunlu Yin, Jiawen Zhan, Minshan Feng, Jie Yu, Wu Sun, Xin Chen · 发表于：Journal of Pharmaceutical and Biomedical Analysis · 年份：2023 · DOI：10.1016/j.jpba.2023.115656 · 被引用次数：8 · 研究领域：Spine and Intervertebral Disc Pathology、Musculoskeletal pain and rehabilitation、Pregnancy-related medical research

Degeneration of the intervertebral disc is primarily caused by the loss of nucleus pulposus cells (NPCs) and extracellular matrix (ECM) (IDD). Bu-Shen-Huo-Xue-Fang (BSHXF), a traditional Chinese medicine decoction, has been used to treat IDD in clinical; nevertheless, the active components and underlying molecular mechanisms remain unknown. BSHXF improved IL-1β and H 2 O 2 stimulation-induced injuries on NPCs by promoting cell viability , increasing ECM deposition, inhibiting cell senescence, and decreasing the levels of inflammatory factors. The active ingredients in BSHXF were identified by LC-MS/MS analysis; three active ingredients from the principal drugs, Aucubin , Tanshinol, and Tanshinone II A promoted NPC viability; and Aucubin and Tanshinol promoted NPC viability more. Aucubin and Tanshinol, respectively, improved H 2 O 2 stimulation-induced injuries on NPCs by promoting cell viability , increasing ECM deposition, inhibiting cell senescence, and decreasing the levels of inflammatory factors. The activator of NF-κB and Wnt signaling pathways attenuated Aucubin and Tanshinol's protective effects by promoting ECM degradation and NPC senescence. Aucubin, Tanshinol, and Tanshinone II A were identified as the most potent compounds in BSHXF protection against degenerative changes in NPCs. The NF-κB and Wnt signaling pathways might be involved in the protective effects of Aucubin and Tanshinol against H 2 O 2 -induced degenerative changes.