Multilayer omics reveals the molecular mechanism of early infection of Clonorchis sinensis juvenile

作者：Yuhong Wu, Xueling Deng, Zhanshuai Wu, Dengyu Liu, Xiaoyin Fu, Lili Tang, Shanshan He, Jiahui Lv, Jilong Wang, Qing Li, Tingzheng Zhan, Zeli Tang · 发表于：Parasites & Vectors · 年份：2023 · DOI：10.1186/s13071-023-05891-1 · 被引用次数：10 · 研究领域：Parasites and Host Interactions、Helminth infection and control、Parasite Biology and Host Interactions

BACKGROUND: Clonorchiasis remains a non-negligible global zoonosis, causing serious socioeconomic burdens in endemic areas. Clonorchis sinensis infection typically elicits Th1/Th2 mixed immune responses during the course of biliary injury and periductal fibrosis. However, the molecular mechanism by which C. sinensis juvenile initially infects the host remains poorly understood. METHODS: The BALB/c mouse model was established to study early infection (within 7 days) with C. sinensis juveniles. Liver pathology staining and observation as well as determination of biochemical enzymes, blood routine and cytokines in blood were conducted. Furthermore, analysis of liver transcriptome, proteome and metabolome changes was performed using multi-omics techniques. Statistical analyses were performed using Student's t-test. RESULTS: Histopathological analysis revealed that liver injury, characterized by collagen deposition and inflammatory cell infiltration, occurred as early as 24 h of infection. Blood indicators including ALT, AST, WBC, CRP and IL-6 indicated that both liver injury and systemic inflammation worsened as the infection progressed. Proteomic data showed that apoptosis and junction-related pathways were enriched within 3 days of infection, indicating the occurrence of liver injury. Furthermore, proteomic and transcriptomic analysis jointly verified that the detoxification and antioxidant defense system was activated by enrichment of glutathione metabolism and cytochrome P450...