Evaluation of the clinical relevance of the Biofire© FilmArray pneumonia panel among hospitalized patients

作者：Kirstine K. Søgaard, Vladimira Hinić, Daniel Goldenberger, Alexander Gensch, Michael Schweitzer, Veronika Bättig, Martin Siegemund, Stefano Bassetti, Roland M Bingisser, Michael Tamm, Manuel Battegay, Maja Weisser, Daiana Stolz, Nina Khanna, Adrian Egli · 发表于：Infection · 年份：2023 · DOI：10.1007/s15010-023-02080-1 · 被引用次数：23 · 研究领域：Nosocomial Infections in ICU、Respiratory viral infections research、Cystic Fibrosis Research Advances

PURPOSE: FilmArray pneumonia (PN)-panel against standard culture in broncho-alveolar lavage (BAL) samples. METHODS: Setting: University Hospital Basel (February 2019 to July 2020), including hospitalized patients with a BAL (± pneumonia). We determined sensitivity and specificity of the PN-panel against standard culture. Using univariate logistic regression, we calculated odds ratios (OR) for pneumonia according to PN-panel and culture status, stratifying by chronic pulmonary disease. We calculated ORs for pneumonia for different pathogens to estimate the clinical relevance. RESULTS: We included 840 adult patients, 60% were males, median age was 68 years, 35% had chronic pulmonary disease, 21% had pneumonia, and 36% had recent antibiotic use. In 1078 BAL samples, bacterial pathogens were detected in 36% and 16% with PN-panel and culture, respectively. The overall sensitivity and specificity of the PN-panel was high, whereas the positive predictive value was low. The OR of pneumonia was 1.1 (95% CI 0.7-1.6) for PN-panel-positive only; 2.6 (95% CI 1.3-5.3) for culture-positive only, and 1.6 (95% CI 1.0-2.4) for PN-panel and culture-positive. The detection rate of Haemophilus influenzae, Staphylococcus aureus, and Moraxella catarrhalis in the PN-panel was high but not associated with pneumonia. CONCLUSION: While sensitivity and specificity of PN-panel are high compared to culture, pathogen detection did not correlate well with a pneumonia diagnosis. Patients with culture-positiv...