Functional characterization and allelic mining of OsGLR genes for potential uses in rice improvement

作者：Wei Zeng, Hua Li, Fanlin Zhang, Xinchen Wang, Shamsur Rehman, Shiji Huang, Chenyang Zhang, Fengcai Wu, Jianfeng Li, Yamei Lv, Chaopu Zhang, Min Li, Zhikang Li, Yingyao Shi · 发表于：Frontiers in Plant Science · 年份：2023 · DOI：10.3389/fpls.2023.1236251 · 被引用次数：10 · 研究领域：Plant and Biological Electrophysiology Studies、GABA and Rice Research、Plant Molecular Biology Research

Glutamate-like receptor (GLR) genes are a group of regulatory genes involved in many physiological processes of plants. With 26 members in the rice genome, the functionalities of most rice GLR genes remain unknown. To facilitate their potential uses in rice improvement, an integrated strategy involving CRISPR-Cas9 mediated knockouts, deep mining and analyses of transcriptomic responses to different abiotic stresses/hormone treatments and gene CDS haplotype (gcHap) diversity in 3,010 rice genomes was taken to understand the functionalities of the 26 rice GLR genes, which led us to two conclusions. First, the expansion of rice GLR genes into a large gene family during evolution had gone through repeated gene duplication events occurred primarily in two large GLR gene clusters on rice chromosomes 9 and 6, which was accompanied with considerable functional differentiation. Secondly, except for two extremely conserved ones ( OsGLR6.2 and OsGLR6.3 ), rich gcHap diversity exists at the remaining GLR genes which played important roles in rice population differentiation and rice improvement, evidenced by their very strong sub-specific and population differentiation, by their differentiated responses to day-length and different abiotic stresses, by the large phenotypic effects of five GLR gene knockout mutants on rice yield traits, by the significant association of major gcHaps at most GLR loci with yield traits, and by the strong genetic bottleneck effects and artificial selection on ...