Infection of sheep by Echinococcus multilocularis in Gansu, China: evidence from mitochondrial and nuclear DNA analysis

作者：Nigus Abebe Shumuye, Li Li, Wenhui Li, Nian‐Zhang Zhang, Yan‐Tao Wu, Yao-Dong Wu, Wenjun Tian, Lin-Sheng Zhang, Xiao-Feng Nian, Guo-Dong Dai, Weigang Chen, Sheng-Zhi Gao, Xue‐Qi Tian, Jun-Shi Liu, Bin Li, Nigatu Kebede, Bao‐Quan Fu, Hongbin Yan, Wan‐Zhong Jia · 发表于：Infectious Diseases of Poverty · 年份：2023 · DOI：10.1186/s40249-023-01120-0 · 被引用次数：3 · 研究领域：Parasitic infections in humans and animals、Parasitic Infections and Diagnostics、Amoebic Infections and Treatments

BACKGROUND: In the normal life cycle of the parasite (Echinococcus multilocularis) that causes alveolar echinococcosis, domestic and wild carnivores act as definitive hosts, and rodents act as intermediate hosts. The presented study contributes to the research on the distribution and transmission pattern of E. multilocularis in China having identified sheep as an unusual intermediate host taking part in the domestic transmission of alveolar echinococcosis in Gansu Province, China. METHODS: From 2020 to 2021, nine whitish different cyst-like were collected from the liver of sheep in Gansu Province for examination. A near complete mitochondrial (mt) genome and selected nuclear genes were amplified from the cyst-like lesion for identification. To confirm the status of the specimen, comparative analysis with reference sequences, phylogenetic analysis, and network analysis were performed. RESULTS: The isolates displayed ≥ 98.87% similarity to E. multilocularis NADH dehydrogenase sub-unit 1 (nad1) (894 bp) reference sequences deposited in GenBank. Furthermore, amplification of the nad4 and nad2 genes also confirmed all nine samples as E. multilocularis with > 99.30% similarity. Additionally, three nuclear genes, pepck (1545 bp), elp-exons VII and VIII (566 bp), and elp-exon IX (256 bp), were successfully amplified and sequenced for one of the isolates with 98.42% similarity, confirming the isolates were correctly identified as E. multilocularis. Network analysis also correctly plac...