A translational regulator MHZ9 modulates ethylene signaling in rice

作者：Yi‐Hua Huang, Jiaqi Han, Biao Ma, Wu‐Qiang Cao, Xinkai Li, Qing Xiong, He Zhao, Rui Zhao, Xun Zhang, Yang Zhou, Wei Wei, Jian‐Jun Tao, Wan‐Ke Zhang, Wenfeng Qian, Shou‐Yi Chen, Chao Yang, Cui‐Cui Yin, Jin‐Song Zhang · 发表于：Nature Communications · 年份：2023 · DOI：10.1038/s41467-023-40429-0 · 被引用次数：23 · 研究领域：Plant responses to water stress、Postharvest Quality and Shelf Life Management、Plant Stress Responses and Tolerance

Ethylene plays essential roles in rice growth, development and stress adaptation. Translational control of ethylene signaling remains unclear in rice. Here, through analysis of an ethylene-response mutant mhz9, we identified a glycine-tyrosine-phenylalanine (GYF) domain protein MHZ9, which positively regulates ethylene signaling at translational level in rice. MHZ9 is localized in RNA processing bodies. The C-terminal domain of MHZ9 interacts with OsEIN2, a central regulator of rice ethylene signaling, and the N-terminal domain directly binds to the OsEBF1/2 mRNAs for translational inhibition, allowing accumulation of transcription factor OsEIL1 to activate the downstream signaling. RNA-IP seq and CLIP-seq analyses reveal that MHZ9 associates with hundreds of RNAs. Ribo-seq analysis indicates that MHZ9 is required for the regulation of ~ 90% of genes translationally affected by ethylene. Our study identifies a translational regulator MHZ9, which mediates translational regulation of genes in response to ethylene, facilitating stress adaptation and trait improvement in rice.