Immunogenic mapping of rDyn-1 and rKDDR-plus proteins and selection of oligopeptides by immunoblotting for the diagnosis of Leishmania infantum-infected dogs

作者：Williane Fernanda Siqueira, Mariana Santos Cardoso, Vanêssa Gomes Fraga, Jennifer Ottino, Vítor Márcio Ribeiro, Carolina Novato Gondim, Joziana Muniz de Paiva Barçante, Ana Carolina Amado Gomes, Alexsandro Sobreira Galdino, Kasper Eersels, Bart van Grinsven, Daniella Castanheira Bartholomeu, Lilian Lacerda Bueno, Thomas J. Cleij, Ricardo Toshio Fujiwara · 发表于：PLoS neglected tropical diseases · 年份：2023 · DOI：10.1371/journal.pntd.0011535 · 被引用次数：7 · 研究领域：Research on Leishmaniasis Studies、Toxin Mechanisms and Immunotoxins、Vector-borne infectious diseases

Endemic in Brazil, visceral leishmaniasis (VL) is a zoonotic infection that is among the most important parasitic diseases transmitted by vectors. Dogs are the main reservoirs of canine leishmaniasis (CanL) and their identification is used in some countries as part of disease prevention and control measures in the canine and human population. In this context, serological tests are necessary, composed of antigens capable of correctly identifying infected dogs, minimizing the number of false-negative cases. This study aimed to identify more immunoreactive peptides derived from two previously described whole proteins (rDyn-1 and rKDDR-plus) and compare their performance to the control antigens rK39 and the crude extract for the detection of dogs infected with L. infantum, especially the asymptomatic ones. The three selected peptides and a mixture of them, along with the rDyn-1, rKDDR-plus, rK39, and crude extract antigens were evaluated using indirect ELISA with sera samples from 186 dogs with CanL, being asymptomatic (n = 50), symptomatic (n = 50), co-infected (n = 19), infected with Babesia sp. (n = 7), Ehrlichia sp. (n = 6), T. cruzi (n = 20) and uninfected (n = 34). The results showed that the rDyn-1 protein and the peptide mixture had the highest sensitivity (100% and 98.32%, respectively) and specificity (97.01 and 98.51, respectively). A high degree of kappa agreement was found for rDyn-1 protein (0.977), mixed peptides (0.965), rKDDR-plus protein (0.953), K-plus peptide ...