Genomic traits of multidrug resistant enterotoxigenic Escherichia coli isolates from diarrheic pigs

作者：Jiameng Hu, Junlin Li, Xiao-Bo Huang, Jing Xia, Min Cui, Yong Huang, Yiping Wen, Yue Xie, Qin Zhao, Sanjie Cao, Likou Zou, Xinfeng Han · 发表于：Frontiers in Microbiology · 年份：2023 · DOI：10.3389/fmicb.2023.1244026 · 被引用次数：17 · 研究领域：Escherichia coli research studies、Antibiotic Resistance in Bacteria、Salmonella and Campylobacter epidemiology

Diarrhea caused by enterotoxigenic Escherichia coli (ETEC) infections poses a significant challenge in global pig farming. To address this issue, the study was conducted to identify and characterize 19 ETEC isolates from fecal samples of diarrheic pigs sourced from large-scale farms in Sichuan Province, China. Whole-genome sequencing and bioinformatic analysis were utilized for identification and characterization. The isolates exhibited substantial resistance to cefotaxime, ceftriaxone, chloramphenicol, ciprofloxacin, gentamicin, ampicillin, tetracycline, florfenicol, and sulfadiazine, but were highly susceptible to amikacin, imipenem, and cefoxitin. Genetic diversity among the isolates was observed, with serotypes O22:H10, O163orOX21:H4, and O105:H8 being dominant. Further analysis revealed 53 resistance genes and 13 categories of 195 virulence factors. Of concern was the presence of tet (X4) in some isolates, indicating potential public health risks. The ETEC isolates demonstrated the ability to produce either heat-stable enterotoxin (ST) alone or both heat-labile enterotoxin (LT) and ST simultaneously, involving various virulence genes. Notably, STa were linked to human disease. Additionally, the presence of 4 hybrid ETEC/STEC isolates harboring Shiga-like toxin-related virulence factors, namely stx2a , stx2b , and stx2e-ONT-2771 , was identified. IncF plasmids carrying multiple antimicrobial resistance genes were prevalent, and a hybrid ETEC/STEC plasmid was detected, hig...