Alterations of microbiota and metabolites in the feces of calves with diarrhea associated with rotavirus and coronavirus infections

作者：Shengwei Cui, Shihui Guo, Qingmei Zhao, Yong Li, Yun Ma, Yongtao Yu · 发表于：Frontiers in Microbiology · 年份：2023 · DOI：10.3389/fmicb.2023.1159637 · 被引用次数：21 · 研究领域：Viral gastroenteritis research and epidemiology、Animal health and immunology

The changes in the composition of intestinal microbiota and metabolites have been linked to digestive disorders in calves, especially neonatal calf diarrhea. Bovine rotavirus (BRV) and bovine coronavirus (BCoV) are known to be the primary culprits behind neonatal calf diarrhea. In this study, we analyzed changes in the fecal microbiota and metabolites of calves with neonatal diarrhea associated with BRV and BCoV infection using high-throughput 16S rRNA sequencing and metabolomics technology. The microbial diversity in the feces of calves infected with BRV and BCoV with diarrhea decreased significantly, and the composition changed significantly. The significant increase of Fusobacterium and the reductions of some bacteria genera, including Faecalibacterium , Bifidobacterium , Ruminococcus , Subdoligranulum , Parabacteroides , Collinsella, and Olsenella , etc., were closely related to diarrhea associated with BRV and BCoV infection. Metabolites in the feces of BRV and BCoV-infected calves with diarrhea were significantly changed. Phosphatidylcholine [PC; 16:1(9 Z)/16:1(9 Z)], lysophosphatidylethanolamine (LysoPE; 0:0/22:0), lysophosphatidylcholine (LysoPC; P-16:0) and LysoPE (0:0/18:0) were significantly higher in the feces of BRV-infected calves with diarrhea. In contrast, some others, such as desthiobiotin, were significantly lower. BRV infection affects glycerophospholipid metabolism and biotin metabolism in calves. Two differential metabolites were significantly increased, ...