Integrated transcriptomics and proteomics assay identifies the role of FCGR1A in maintaining sperm fertilization capacity during semen cryopreservation in sheep

作者：Jiachen Bai, Guizhen Zhou, Shaopeng Hao, Yucheng Liu, Yanhua Guo, Jingjing Wang, Hongtao Liu, Longfei Wang, Jun Li, Aiju Liu, Wendell Q. Sun, Pengcheng Wan, Xiangwei Fu · 发表于：Frontiers in Cell and Developmental Biology · 年份：2023 · DOI：10.3389/fcell.2023.1177774 · 被引用次数：10 · 研究领域：Sperm and Testicular Function、Reproductive Biology and Fertility、Selenium in Biological Systems

Semen cryopreservation is a promising technology employed in preserving high-quality varieties in animal husbandry and is also widely applied in the human sperm bank. However, the compromised qualities, such as decreased sperm motility, damaged membrane structure, and reduced fertilization competency, have significantly hampered the efficient application of this technique. Therefore, it is imperative to depict various molecular changes found in cryopreserved sperm and identify the regulatory network in response to the cryopreservation stress. In this study, semen was collected from three Chinese Merino rams and divided into untreated (fresh semen, FS) and programmed freezing (programmed freezing semen, PS) groups. After measuring different quality parameters, the ultra-low RNA-seq and tandem mass tag-based (TMT) proteome were conducted in both the groups. The results indicated that the motility (82.63% ± 3.55% vs. 34.10% ± 2.90%, p < 0.05) and viability (89.46% ± 2.53% vs. 44.78% ± 2.29%, p < 0.05) of the sperm in the FS group were significantly higher compared to those in the PS group. In addition, 45 upregulated and 291 downregulated genes, as well as 30 upregulated and 48 downregulated proteins, were found in transcriptomics and proteomics data separately. Moreover, three integrated methods, namely, functional annotation and enrichment analysis, Pearson’s correlation analysis, and two-way orthogonal partial least squares (O2PLS) analysis, were used for furthe...