A specific and low background nucleic acids sensing strategy based on rolling circle amplification coupled with a magnetic DNA machine

作者：Xiangde Lai, Xuan Zhao, Yanan Peng, Rui Zhang, Huajie Pang, Zhijun Gao, Dongxia Li, Peipei Cao, Qiumei Pu, Bin Qiao, Hua Pei, Qiang Wu · 发表于：Chemical Communications · 年份：2023 · DOI：10.1039/d3cc02838k · 被引用次数：11 · 研究领域：Advanced biosensing and bioanalysis techniques、Plasmonic and Surface Plasmon Research、Monoclonal and Polyclonal Antibodies Research

We propose a universal fluorescence method for detection of nucleic acids based on rolling circle amplification (RCA) combined with a magnetic DNA machine and using dengue virus nucleic acids as an example target. RCA specifically amplifies the target and yields a large number of initiators employing heat-labile double-stranded DNase. The magnetic DNA machine produces a fluorescence signal and eliminates background noise. This method achieved a wide linear range, promising recovery and ultrahigh recognition specificity for one-base mismatches, and indicates the potential application of this sensing strategy in the clinical diagnosis of nucleic acids of pathogens.