Transcriptome and the gut microbiome analysis of the impacts of Brucella abortus oral infection in BALB/c mice

作者：Kun Han, Hao Dong, Xiaowei Peng, Jiali Sun, Hui Jiang, Yu Feng, Jiabo Ding, Sa Xiao · 发表于：Microbial Pathogenesis · 年份：2023 · DOI：10.1016/j.micpath.2023.106278 · 被引用次数：4 · 研究领域：Brucella: diagnosis, epidemiology, treatment、Salmonella and Campylobacter epidemiology、Escherichia coli research studies

Brucellosis is a zoonotic infectious disease caused by Brucella spp, which could cause serious economic losses to animal husbandry and threaten human public health. Ingestion of contaminated animal products is a common way to acquire Brucella infection in humans, while research on effect of oral Brucella infection on host gut microbiota and the gene expression in intestinal tissues is limited. In the present study, 16S rRNA sequencing and RNA sequencing were conducted to explore gut microbiota and expression profiles of mRNAs in the colon of BALB/c mice, which were infected by Brucella abortus 2308. The fecal samples were collected at 7 and 28 days post infection to observe changes in the gut microbiota during Brucella infection. In the alpha diversity analysis, significantly increased Chao 1 index was observed at 28 days after Brucella infection. The Bray-Curtis distancebased principal coordinate analysis indicated that the WT group showed a separation from the Brucella infection groups. In addition, analysis of composition of microbes revealed that Prevotellaceae_NK3B31_group were more abundant in 1 week and 4 week infection groups, while Turicibacter was only more abundant in 4 week infection group. Based on the RNA-seq assay, a total of 45 differentially expressed genes were detected between Brucella abortus infection group and control group. Furthermore, KEGG pathway enrichment analysis showed that protein processing in endoplasmic reticulum, Legionellosis, Spliceosome, ...