Single-cell microfluidics in combination with chlorophyll a fluorescence measurements to assess the lifetime of the Chlamydomonas PSBO protein

作者：Éva Széles, Soujanya Kuntam, André Vidal‐Meireles, V.Yu. Nagy, Krisztina Nagy, Ágnes Ábrahám, László Kovács, Szilvia Z. Tóth · 发表于：Photosynthetica · 年份：2023 · DOI：10.32615/ps.2023.028 · 被引用次数：12 · 研究领域：Photosynthetic Processes and Mechanisms、Photoreceptor and optogenetics research、Advanced Fluorescence Microscopy Techniques

PSBO is an essential subunit of the oxygen-evolving complex and we recently demonstrated that its lifetime depends on environmental conditions in Chlamydomonas reinhardtii . To assess PSBO lifetime with a high time resolution, we employed ( 1 ) a microfluidic platform enabling the trapping of single cells and the parallel measurement of photosynthetic activity, and ( 2 ) a nitrate-inducible PSBO amiRNA line. Our microfluidic platform allowed the rapid replacement of the nutrient solution necessary for induction. It also enabled the precise monitoring of the decline in the F v /F m value, reflecting PSBO loss. We found that in the dark, at medium and high light intensity, the F v /F m value decreased with halftimes of about 25, 12.5, and 5 h, respectively. We also observed that photosynthetic activity was better sustained upon carbon limitation. In the absence of acetate, the halftimes of F v /F m diminishment doubled to quadrupled compared with the control, acetate-supplied cultures.