Genome−wide expression profile analysis of the NHE and NKA gene family in Rachycentron canadum (Linnaeus, 1766) and its response to salinity adaptation

作者：Zongfa Chen, Baosong Huang, Ziqi Yan, Yujie Hong, Mingming Zhao, Minxuan Jin, Anna Zheng, Zhong‐Liang Wang · 发表于：Frontiers in Marine Science · 年份：2023 · DOI：10.3389/fmars.2023.1228933 · 被引用次数：5 · 研究领域：Physiological and biochemical adaptations、Aquaculture Nutrition and Growth、Fish Ecology and Management Studies

NHE and NKA are important regulators of ion transport in fish and play a pivotal role in maintaining osmotic balance and adapting to salinity changes. However, no systematic identification and functional analysis has been conducted for NHEs and NKAs in the cobia ( Rachycentron canadum ), a commercially important worldwide flatfish. Herein,12 NHE genes were found to be distributed on 10 chromosomes and 12 NKA genes were found to be distributed on 9 chromosomes were identified in the R. canadum at the genome-wide level. Histopathological examination of the gills demonstrated the response of gill lamellae and chloride cells to salinity, while the microstructure of the intestine and kidney exhibited changes associated with salinity. The findings show that members of the NHE and NKA gene families are widely distributed in gill, brain, and heart tissues. Specifically, NHE genes exhibited high expression levels in the gill, somatic kidney, and brain, whereas NKA genes displayed prominent expression in the gill, brain, and heart. Moreover, salinity adaptation experiments were conducted to examine the response of NHE and NKA genes. In the intestine, NHE1 expression was significantly upregulated following both high and low salt stimulation, while the somatic kidney exhibited a proportional response to changes in salinity. Notably, a significant downward trend in NHE2c expression was observed in the gill, intestine, and somatic kidney with increasing salinity. Following low-salt acclima...