Description of lipase producing novel yeast species Debaryomyces apis f.a., sp. nov. and a modified pH indicator dye-based method for the screening of lipase producing microorganisms

作者：Alka Kumari, Kanti N. Mihooliya, Debendra K. Sahoo, Mani Shankar Bhattacharyya, G. S. Prasad, Anil Kumar Pinnaka · 发表于：Scientific Reports · 年份：2023 · DOI：10.1038/s41598-023-38241-3 · 被引用次数：8 · 研究领域：Fermentation and Sensory Analysis、Yeasts and Rust Fungi Studies、Fungal and yeast genetics research

Abstract Four yeast strains were isolated from the gut of stingless bee, collected in Churdhar, Himachal Pradesh, India. Physiological characterization, morphological examination, and sequence analysis of small subunit ribosomal RNA (18S rRNA) genes, internal transcribed spacer (ITS) region, and D1/D2 domain of the large subunit rRNA gene revealed that the four strains isolated from the gut of stingless bee belonged to the Debaryomyces clade. Strain CIG-23H T showed sequence divergence of 7.5% from Debaryomyces nepalensis JCM 2095 T , 7.8% from Debaryomyces udenii JCM 7855 T , and Debaryomyces coudertii JCM 2387 T in the D1/D2 domain. In the ITS region sequences, strain CIG-23H T showed a 15% sequence divergence from Debaryomyces nepalensis JCM 2095 T and Debaryomyces coudertii JCM 2387 T . In 18S rRNA gene sequence, the strain CIG-23H T showed 1.14% sequence divergence from Debaryomyces nepalensis JCM 2095 and and Debaryomyces coudertii JCM 2387, and 0.83% sequence divergence from Debaryomyces hansenii NRRL Y-7426. Strain CIG-23H T can utilize more carbon sources than closely related species. The findings suggest that strain CIG-23H T is a novel species of the genus Debaryomyces , and we propose to name it as Debaryomyces apis f.a., sp. nov. The holotype is CBS 16297 T , and the isotypes are MTCC 12914 T and KCTC 37024 T . The MycoBank number of Debaryomyces apis f.a., sp. nov . is MB836065. Additionally, a method using cresol red and Bromothymol blue pH indicator dyes was d...