Application of spatial transcriptomics analysis using the Visium system for the mouse nasal cavity after intranasal vaccination

作者：Sakiko Toyama, Tomoko Honda, Sadahiro Iwabuchi, Shinichi Hashimoto, Kenzaburo Yamaji, Yuko Tokunaga, Yusuke Matsumoto, Hideya Kawaji, Takashi Miyazaki, Yoshiaki Kikkawa, Michinori Kohara · 发表于：Frontiers in Immunology · 年份：2023 · DOI：10.3389/fimmu.2023.1209945 · 被引用次数：12 · 研究领域：Immune Response and Inflammation、Immunotherapy and Immune Responses、Influenza Virus Research Studies

Intranasal vaccines that elicit mucosal immunity are deemed effective against respiratory tract infections such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), but their ability to induce humoral immunity characterized by immunoglobulin A (IgA) and IgG production is low. It has been reported that vaccination with a mixture of a viscous base carboxyvinyl polymer (CVP) and viral antigens induced robust systemic and mucosal immune responses. In this study, we analyzed the behavior of immunocompetent cells in the nasal cavity over time by spatial transcriptome profiling induced immediately after antigen vaccination using CVP. We established a method for performing spatial transcriptomics using the Visium system in the mouse nasal cavity and analyzed gene expression profiles within the nasal cavity after intranasal vaccination. Glycoprotein 2 (Gp2)-, SRY-box transcription factor 8 (Sox8)-, or Spi-B transcription factor (Spib)-expressing cells were increased in the nasal passage (NP) region at 3–6 hr after SARS-CoV-2 spike protein and CVP (S-CVP) vaccination. The results suggested that microfold (M) cells are activated within a short period of time (3–6 hr). Subsequent cluster analysis of cells in the nasal cavity showed an increase in Cluster 9 at 3–6 hr after intranasal vaccination with the S-CVP. We found that Il6 in Cluster 9 had the highest log2 fold values within the NP at 3–6 hr. A search for gene expression patterns similar to that of Il6 revealed that the ...