L-carnitine promotes liver regeneration after hepatectomy by enhancing lipid metabolism

作者：Xi Zhou, Guobin Huang, Lu Wang, Yuanyuan Zhao, Junbo Li, Dong Chen, Lai Wei, Zhishui Chen, Bo Yang · 发表于：Journal of Translational Medicine · 年份：2023 · DOI：10.1186/s12967-023-04317-x · 被引用次数：21 · 研究领域：Organ Transplantation Techniques and Outcomes、Metabolism and Genetic Disorders、Mitochondrial Function and Pathology

BACKGROUND: Lipid metabolism plays an important role in liver regeneration, but its regulation still requires further research. In this study, lipid metabolites involved in mouse liver regeneration at different time points were sequenced and analyzed to study their influence on liver regeneration and its mechanism. METHODS: Our experiment was divided into two parts. The first part examined lipid metabolites during liver regeneration in mice. In this part, lipid metabolites were sequentially analyzed in the livers of 70% mouse hepatectomy models at 0, 1, 3and 7 days after operation to find the changes of lipid metabolites in the process of liver regeneration. We screened L-carnitine as our research object through metabolite detection. Therefore, in the second part, we analyzed the effects of carnitine on mouse liver regeneration and lipid metabolism during liver regeneration. We divided the mouse into four groups: control group (70% hepatectomy group); L-carnitine group (before operation) (L-carnitine were given before operation); L-carnitine group (after operation)(L-carnitine were given after operation) and L-carnitine + perhexiline maleate (before operation) group. Weighing was performed at 24 h, 36 and 48 h in each group, and oil red staining, HE staining and MPO staining were performed. Tunnel fluorescence staining, Ki67 staining and serological examination. RESULTS: Sequencing analysis of lipid metabolites in 70% of mouse livers at different time points after hepatectomy...