Multistage Anticoagulant Surfaces: A Synergistic Combination of Protein Resistance, Fibrinolysis, and Endothelialization

作者：Jian Feng, Jinghong Wang, Huanhuan Wang, Xinyin Cao, Xiaoliang Ma, Yu Rao, Huimin Pang, Sulei Zhang, Yuheng Zhang, Lei Wang, Xiaoli Liu, Hong Chen · 发表于：ACS Applied Materials & Interfaces · 年份：2023 · DOI：10.1021/acsami.3c05145 · 被引用次数：24 · 研究领域：Blood properties and coagulation、Platelet Disorders and Treatments、Blood Coagulation and Thrombosis Mechanisms

Anticoagulant surface modification of blood-contacting materials has been shown to be effective in preventing thrombosis and reducing the dose of anticoagulant drugs that patients take. However, commercially available anticoagulant coatings, that is, both bioinert and bioactive coatings, are typically based on a single anticoagulation strategy. This puts the anticoagulation function of the coating at risk of failure during long-term use. Considering the several pathways of the human coagulation system, the synergy of multiple anticoagulation theories may provide separate, targeted effects at different stages of thrombosis. Based on this presumption, in this work, negatively charged poly(sodium p -styrenesulfonate- co -oligo(ethylene glycol) methyl ether methacrylate) and positively charged poly(lysine- co -1-adamantan-1-ylmethyl methacrylate) were synthesized to construct matrix layers on the substrate by electrostatic layer-by-layer self-assembly (LBL). Amino-functionalized β-cyclodextrin (β-CD-PEI) was subsequently immobilized on the surface by host–guest interactions, and heparin was grafted. By adjusting the content of poly(oligo(ethylene glycol) methyl ether methacrylate) (POEGMA), the interactions between modified surfaces and plasma proteins/cells were regulated. This multistage anticoagulant surface exhibits inertness at the initial stage of implantation, resisting nonspecific protein adsorption (POEGMA). When coagulation reactions occur, heparin exerts its active ant...