Effects of different forage proportions in fermented total mixed ration on muscle fatty acid profile and rumen microbiota in lambs

作者：Mingjian Liu, Zhijun Wang, Lin Sun, Yu Wang, Junfeng Li, Gentu Ge, Yushan Jia, Shuai Du · 发表于：Frontiers in Microbiology · 年份：2023 · DOI：10.3389/fmicb.2023.1197059 · 被引用次数：12 · 研究领域：Ruminant Nutrition and Digestive Physiology、Animal Nutrition and Health、Animal Nutrition and Physiology

Objective The objectives of this study were to evaluate the effects of different forage proportions in the fermented total mixed ration (FTMR) on growth performance, muscle fatty acid profile, and rumen microbiota of lambs. Methods Thirty 6-month-old small tail Han sheep × Ujumqin lambs with initial body weight (BW) of 27.8 ± 0.90 kg were selected for the test and divided into two groups of 15 sheep in each treatment (three pens per treatment and five lambs per pen) according to the principle of homogeneity. Two isoenergetic and isonitrogenous diets were formulated according to the NRC. The diet treatments were designed as (1) OH treatment containing 25% alfalfa hay and 35% oat hay, and (2) AH treatment containing 35% alfalfa hay with 25% oat hay. The forage-to-concentrate ratio for both diets was 65: 35 (DM basis). Three replicates were randomly selected from each treatment to determine growth performance, fatty acid profile and rumen bacterial communities in lambs. Results Results revealed no statistically significant ( p > 0.05) differences in dry matter intake and average daily gain between the two diet groups. Cholesterol and intramuscular fat were significantly ( p > 0.05) higher in the AH group, while no statistically significant difference ( p > 0.05) was found in pH24 value. The muscle fatty acid compositions of lambs were obviously ( p < 0.05) influenced by the diet treatments. Compared with the OH group, the C16:1, C17:0, and C20:3n6 con...