Hydroxylases involved in terpenoid biosynthesis: a review

作者：Zihan Zhang, Qing‐Yang Wu, Yue Ge, Zhengyu Huang, Ran Hong, Aitao Li, Jian‐He Xu, Hui‐Lei Yu · 发表于：Bioresources and Bioprocessing · 年份：2023 · DOI：10.1186/s40643-023-00656-1 · 被引用次数：55 · 研究领域：Plant biochemistry and biosynthesis、Photosynthetic Processes and Mechanisms、Natural product bioactivities and synthesis

Terpenoids are pervasive in nature and display an immense structural diversity. As the largest category of plant secondary metabolites, terpenoids have important socioeconomic value in the fields of pharmaceuticals, spices, and food manufacturing. The biosynthesis of terpenoid skeletons has made great progress, but the subsequent modifications of the terpenoid framework are poorly understood, especially for the functionalization of inert carbon skeleton usually catalyzed by hydroxylases. Hydroxylase is a class of enzymes that plays an important role in the modification of terpenoid backbone. This review article outlines the research progress in the identification, molecular modification, and functional expression of this class of enzymes in the past decade, which are profitable for the discovery, engineering, and application of more hydroxylases involved in the plant secondary metabolism.