African swine fever virus protein p17 promotes mitophagy by facilitating the interaction of SQSTM1 with TOMM70

作者：Boli Hu, Guifang Zhong, Shuxiang Ding, Kang Xu, Xiran Peng, Wei-Ren Dong, Jiyong Zhou · 发表于：Virulence · 年份：2023 · DOI：10.1080/21505594.2023.2232707 · 被引用次数：27 · 研究领域：Animal Disease Management and Epidemiology、T-cell and Retrovirus Studies、Viral Infections and Vectors

Viruses have developed different strategies to hijack mitophagy to facilitate their replication. However, whether and how African swine fever virus (ASFV) regulates mitophagy are largely unknown. Here, we found that the ASFV-encoded p17 induced mitophagy. Coimmunoprecipitation/mass spectrometry assays identified translocase of outer mitochondrial membrane 70 (TOMM70) as the protein that interacted with p17. The binding of TOMM70 to p17 promoted the binding of the mitophagy receptor SQSTM1 to TOMM70, led to engulfment of mitochondria by autophagosomes, and consequently decreased the number of mitochondria. Consistently, the levels of TOMM70 and TOMM20 decreased substantially after p17 expression or ASFV infection. Furthermore, p17-mediated mitophagy resulted in the degradation of mitochondrial antiviral signalling proteins and inhibited the production of IFN-α, IL-6 and TNFα. Overall, our findings suggest that ASFV p17 regulates innate immunity by inducing mitophagy via the interaction of SQSTM1 with TOMM70.