Differences in the lipid metabolism profile and clinical characteristics between eosinophilic and non-eosinophilic acute exacerbation of chronic obstructive pulmonary disease

作者：Yating Wang, Chun Chang, Sifan Tian, Juan Wang, Xiaoyan Gai, Qiqiang Zhou, Yahong Chen, Xu Gao, Yongchang Sun, Ying Liang · 发表于：Frontiers in Molecular Biosciences · 年份：2023 · DOI：10.3389/fmolb.2023.1204985 · 被引用次数：9 · 研究领域：Chronic Obstructive Pulmonary Disease (COPD) Research、Metabolomics and Mass Spectrometry Studies、High Altitude and Hypoxia

Objective: In this study, we aimed to investigate the differences in serum lipid metabolite profiles and their relationship with clinical characteristics between patients with eosinophilic and non-eosinophilic AECOPD. Methods: A total of 71 AECOPD patients were enrolled. Eosinophilic AECOPD was defined as blood EOS% ≥ 2% ( n = 23), while non-eosinophilic AECOPD, as blood EOS< 2% ( n = 48). Clinical data were collected, and serum lipid metabolism profiles were detected by liquid chromatography–mass spectrometry (LC-MS). The XCMS software package was used to pre-process the raw data, and then, lipid metabolite identification was achieved through a spectral match using LipidBlast library. Differences in lipid profiles and clinical features between eosinophilic and non-eosinophilic groups were analyzed by generalized linear regression. The least absolute shrinkage and selection operator (LASSO) was applied to screen the most characteristic lipid markers for the eosinophilic phenotype. Results: Eosinophilic AECOPD patients had less hypercapnic respiratory failures, less ICU admissions, a shorter length of stay in the hospital, and a lower fibrinogen level. In the lipid metabolism profiles, 32 significantly different lipid metabolites were screened through a t -test adjusted by using FDR (FDR-adjusted p < 0.05 and VIP> 1). Nine differential lipid metabolites were found to be associated with the three clinical features, namely, hypercapnia respiratory failure, I...