A cooperative network at the nuclear envelope counteracts LINC-mediated forces during oogenesis in C. elegans

作者：Chenshu Liu, Rachel Rex, Zoe Lung, John S. Wang, Fan Wu, Hyung Jun Kim, Liangyu Zhang, Lydia L. Sohn, Abby F. Dernburg · 发表于：Science Advances · 年份：2023 · DOI：10.1126/sciadv.abn5709 · 被引用次数：8 · 研究领域：Genetics, Aging, and Longevity in Model Organisms、Nuclear Structure and Function、Mitochondrial Function and Pathology

, oocyte nuclei lacking the single lamin protein LMN-1 are vulnerable to collapse under forces mediated through LINC (linker of nucleoskeleton and cytoskeleton) complexes. Here, we use cytological analysis and in vivo imaging to investigate the balance of forces that drive this collapse and protect oocyte nuclei. We also use a mechano-node-pore sensing device to directly measure the effect of genetic mutations on oocyte nuclear stiffness. We find that nuclear collapse is not a consequence of apoptosis. It is promoted by dynein, which induces polarization of a LINC complex composed of Sad1 and UNC-84 homology 1 (SUN-1) and ZYGote defective 12 (ZYG-12). Lamins contribute to oocyte nuclear stiffness and cooperate with other inner nuclear membrane proteins to distribute LINC complexes and protect nuclei from collapse. We speculate that a similar network may protect oocyte integrity during extended oocyte arrest in mammals.