A reactive oxygen species–related signature to predict prognosis and aid immunotherapy in clear cell renal cell carcinoma

作者：Hongxiang Liu, Yong Luo, Shankun Zhao, Jing Tan, Minjian Chen, Xihai Liu, Jian‐Heng Ye, Shanghua Cai, Yulin Deng, Jinchuang Li, Huichan He, Xin Zhang, Weide Zhong · 发表于：Frontiers in Oncology · 年份：2023 · DOI：10.3389/fonc.2023.1202151 · 被引用次数：4 · 研究领域：Ferroptosis and cancer prognosis、Renal cell carcinoma treatment、Immune cells in cancer

Background: Clear cell renal cell carcinoma (ccRCC) is a malignant disease containing tumor-infiltrating lymphocytes. Reactive oxygen species (ROS) are present in the tumor microenvironment and are strongly associated with cancer development. Nevertheless, the role of ROS-related genes in ccRCC remains unclear. Methods: We describe the expression patterns of ROS-related genes in ccRCC from The Cancer Genome Atlas and their alterations in genetics and transcription. An ROS-related gene signature was constructed and verified in three datasets and immunohistochemical staining (IHC) analysis. The immune characteristics of the two risk groups divided by the signature were clarified. The sensitivity to immunotherapy and targeted therapy was investigated. Results: Our signature was constructed on the basis of glutamate-cysteine ligase modifier subunit (GCLM), interaction protein for cytohesin exchange factors 1 (ICEF1), methionine sulfoxide reductase A (MsrA), and strawberry notch homolog 2 (SBNO2) genes. More importantly, protein expression levels of GCLM, MsrA, and SBNO2 were detected by IHC in our own ccRCC samples. The high-risk group of patients with ccRCC suffered lower overall survival rates. As an independent predictor of prognosis, our signature exhibited a strong association with clinicopathological features. An accurate nomogram for improving the clinical applicability of our signature was constructed. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses sho...