LncRNA BANCR promotes oral squamous cell carcinoma progression via regulating Rab1A signaling

作者：Weiyu Li, Xiaoqing Xu, Zeyi Ma, Peiqi Shen, Bin Cheng, Juan Xia, Yaoyin Li · 发表于：Journal of Oral Pathology and Medicine · 年份：2023 · DOI：10.1111/jop.13463 · 被引用次数：2 · 研究领域：Cancer-related molecular mechanisms research、NF-κB Signaling Pathways、Head and Neck Cancer Studies

Abstract Background Long non‐coding RNA BRAF ‐activated non‐protein coding RNA plays bidirectional roles in human cancers. However, function and molecular mechanism of BRAF ‐activated non‐protein coding RNA in oral squamous cell carcinoma still need to clarify further. Methods Long non‐coding RNA microarray assay, in situ hybridization staining, clinicopathological data analysis were performed to investigate expression pattern of BRAF ‐activated non‐protein coding RNA in oral squamous cell carcinoma tissue samples. Constructing ectopically expressed BRAF ‐activated non‐protein coding RNA in oral squamous cell carcinoma cells via plasmids or siRNAs, then changeable abilities of proliferation and motility of these cells were observed in vitro and in vivo. RNA‐protein pulldown, RNA immunoprecipitation, and bioinformatics analyses were performed to explore potential pathways involved in BRAF ‐activated non‐protein coding RNA‐based regulation of malignant progression in oral squamous cell carcinoma. Results BRAF ‐activated non‐protein coding RNA was identified upregulated in oral squamous cell carcinoma tissue and correlated with nodal metastasis and clinical severity of patients. Overexpressed BRAF ‐activated non‐protein coding RNA increased percentage of 5‐ethynyl‐2′‐deoxyuridine‐positive cells, viability, migration, and invasion rates of oral squamous cell carcinoma cells, while silenced BRAF ‐activated non‐protein coding RNA could observe weakened effects in vitro. Xenograft t...