Genome-wide systematic characterization of the NRT2 gene family and its expression profile in wheat (Triticum aestivum L.) during plant growth and in response to nitrate deficiency

作者：Qingyan Deng, Jiangtao Luo, Jianmin Zheng, Wenfang Tan, Zongjun Pu, Fang Wang · 发表于：BMC Plant Biology · 年份：2023 · DOI：10.1186/s12870-023-04333-5 · 被引用次数：22 · 研究领域：Plant nutrient uptake and metabolism、Plant Micronutrient Interactions and Effects、Nitrogen and Sulfur Effects on Brassica

Abstract Background Wheat ( Triticum aestivum L.) is a major cereal crop that is grown worldwide, and it is highly dependent on sufficient N supply. The molecular mechanisms associated with nitrate uptake and assimilation are still poorly understood in wheat. In plants, NRT2 family proteins play a crucial role in NO 3 – acquisition and translocation under nitrate limited conditions. However, the biological functions of these genes in wheat are still unclear, especially their roles in NO 3 – uptake and assimilation. Results In this study, a comprehensive analysis of wheat TaNRT2 genes was conducted using bioinformatics and molecular biology methods, and 49 TaNRT2 genes were identified. A phylogenetic analysis clustered the TaNRT2 genes into three clades. The genes that clustered on the same phylogenetic branch had similar gene structures and nitrate assimilation functions. The identified genes were further mapped onto the 13 wheat chromosomes, and the results showed that a large duplication event had occurred on chromosome 6. To explore the TaNRT2 gene expression profiles in wheat, we performed transcriptome sequencing after low nitrate treatment for three days. Transcriptome analysis revealed the expression levels of all TaNRT2 genes in shoots and roots, and based on the expression profiles, three highly expressed genes ( TaNRT2-6A.2 , TaNRT2-6A.6 , and TaNRT2-6B.4 ) were selected for qPCR analysis in two different wheat cultivars (‘Mianmai367’ and ‘Nanmai660’) under nitrate-...