PCID2 Subunit of the Drosophila TREX-2 Complex Has Two RNA-Binding Regions

作者：Y. A. Vdovina, М. М. Куршакова, Sofia G. Georgieva, Daria V. Kopytova · 发表于：Current Issues in Molecular Biology · 年份：2023 · DOI：10.3390/cimb45070357 · 被引用次数：7 · 研究领域：RNA Research and Splicing、Nuclear Structure and Function、RNA modifications and cancer

Drosophila PCID2 is a subunit of the TREX-2 mRNA nuclear export complex. Although the complex has long been studied in eukaryotes, it is still unclear how TREX-2 interacts with mRNA in multicellular organisms. Here, the interaction between Drosophila PCID2 and the ras2 RNA was studied by EMSA. We show that the C-terminal region of the WH domain of PCID2 specifically binds the 3′-noncoding region of the ras2 RNA. While the same region of PCID2 interacts with the Xmas-2 subunit of the TREX-2 complex, PCID2 interacts with RNA independently of Xmas-2. An additional RNA-binding region (M region) was identified in the N-terminal part of the PCI domain and found to bind RNA nonspecifically. Point mutations of evolutionarily conserved amino acid residues in this region completely abolish the PCID2–RNA interaction, while a deletion of the C-terminal domain only partly decreases it. Thus, the specific interaction of PCID2 with RNA requires nonspecific PCID2–RNA binding.