Rice stripe virus nonstructural protein 3 suppresses plant defence responses mediated by the MEL – SHMT1 module

作者：Kun Wang, Shuai Fu, Liang Wu, Jianxiang Wu, Yaqin Wang, Yi Xu, Xueping Zhou · 发表于：Molecular Plant Pathology · 年份：2023 · DOI：10.1111/mpp.13373 · 被引用次数：12 · 研究领域：Plant Virus Research Studies、Plant-Microbe Interactions and Immunity、Plant nutrient uptake and metabolism

Our previous study identified an evolutionarily conserved C4HC3-type E3 ligase, named microtubule-associated E3 ligase (MEL), that regulates broad-spectrum plant resistance against viral, fungal and bacterial pathogens in multiple plant species by mediating serine hydroxymethyltransferase (SHMT1) degradation via the 26S proteasome pathway. In the present study, we found that NS3 protein encoded by rice stripe virus could competitively bind to the MEL substrate recognition site, thereby inhibiting MEL interacting with and ubiquitinating SHMT1. This, in turn, leads to the accumulation of SHMT1 and the repression of downstream plant defence responses, including reactive oxygen species accumulation, mitogen-activated protein kinase pathway activation, and the up-regulation of disease-related gene expression. Our findings shed light on the ongoing arms race between pathogens and demonstrate how a plant virus can counteract the plant defence response.