Porous SiO2-Based Reactor with Self-Supply of O2 and H2O2 for Synergistic Photo-Thermal/Photodynamic Therapy

作者：Zhengzhao Li, Lianshan Guo, Liqiao Lin, Tongting Wang, Yanqiu Jiang, Jin Yeong Song, Jihua Feng, Jianfeng Huang, Haoyu Li, Zhihao Bai, Wenqi Liu, Jianfeng Zhang · 发表于：International Journal of Nanomedicine · 年份：2023 · DOI：10.2147/ijn.s387505 · 被引用次数：9 · 研究领域：Nanoplatforms for cancer theranostics、Photodynamic Therapy Research Studies、Advanced Nanomaterials in Catalysis

Purpose: Although the combined photo-thermal (PTT) and photodynamic therapy (PDT) of tumors have demonstrated promise as effective cancer therapy, the hypoxic and insufficient H 2 O 2 supply of tumors seriously limits the efficacy of PDT, and the acidic environment reduces the catalytic activity of nanomaterial in the tumor microenvironment. To develop a platform for efficiently addressing these challenges, we constructed a nanomaterial of [email protected] /GOD-MnO 2 -SiO 2 @ [email protected] (AMS) for combination tumor therapy. The treatment effects of AMS were evaluated both in vitro and in vivo. Methods: In this work, Ce6 and hemin were loaded on graphene (GO) through π-π conjugation, and Fc was connected to GO via amide bond. The [email protected] was loaded into SiO 2 , and coated with dopamine. Then, MnO 2 was modified on the SiO 2 . Finally, [email protected] and GOD were fixed to gain AMS. We characterized the morphology, size, and zeta potential of AMS. The oxygen and reactive oxygen species (ROS) production properties of AMS were analyzed. The cytotoxicity of AMS was detected by MTT and calcein-AM/PI assays. The apoptosis of AMS to a tumor cell was estimated with a JC-1 probe, and the ROS level was detected with a 2’,7’-Dichlorodihydrofluorescein diacetate (DCFH-DA) probe. The anticancer efficacy in vivo was analyzed by the changes in the tumor size in different treatment groups. Results: AMS was targeted to the tumor cell and released doxorubicin. It decomposed g...