Nanochannel Electro‐Injection as a Versatile Platform for Efficient RNA/DNA Programming on Dendritic Cells

作者：Jing Liu, Juan Jiang, Caiguanxi Deng, Xinshuo Huang, Shuang Huang, Zhengjie Liu, Yang Jiang, Jingshan Mo, Hui‐jiuan Chen, Ji Wang, Xi Xie · 发表于：Small · 年份：2023 · DOI：10.1002/smll.202303088 · 被引用次数：18 · 研究领域：Nanopore and Nanochannel Transport Studies、Microfluidic and Bio-sensing Technologies、RNA Interference and Gene Delivery

The utilization of dendritic cell (DC) vaccines is a promising approach in cancer immunotherapy, and the modification of DCs for the expression of tumor-associated antigens is critical for successful cancer immunotherapy. A safe and efficient method for delivering DNA/RNA into DCs without inducing maturation is beneficial to achieve successful DC transformation for cell vaccine applications, yet remains challenging. This work presents a nanochannel electro-injection (NEI) system for the safe and efficient delivery of a variety of nucleic acid molecules into DCs. The device is based on track-etched nanochannel membrane as key components, where the nano-sized channels localize the electric field on the cell membrane, enabling lower voltage (<30 V) for cell electroporation. The pulse conditions of NEI are examined so that the transfection efficiency (>70%) and biosafety (viability >85%) on delivering fluorescent dyes, plasmid DNA, messenger RNA, and circular RNA (circRNA) into DC2.4 are optimized. Primary mouse bone marrow DC can also be transfected with circRNA with 68.3% efficiency, but without remarkably affecting cellular viability or inducing DC maturation. These results suggest that NEI can be a safe and efficient transfection platform for in vitro transformation of DCs and possesses a promising potential for developing DC vaccines against cancer.