Metabolic engineering of human gut microbiome: Recent developments and future perspectives

作者：Peishun Li, Stefan Roos, Hao Luo, Boyang Ji, Jens Nielsen · 发表于：Metabolic Engineering · 年份：2023 · DOI：10.1016/j.ymben.2023.06.006 · 被引用次数：32 · 研究领域：Gut microbiota and health、Microbial Metabolic Engineering and Bioproduction、Pancreatic function and diabetes

Many studies have demonstrated that the gut microbiota is associated with human health and disease. Manipulation of the gut microbiota, e.g. supplementation of probiotics, has been suggested to be feasible, but subject to limited therapeutic efficacy. To develop efficient microbiota-targeted diagnostic and therapeutic strategies, metabolic engineering has been applied to construct genetically modified probiotics and synthetic microbial consortia. This review mainly discusses commonly adopted strategies for metabolic engineering in the human gut microbiome, including the use of in silico, in vitro, or in vivo approaches for iterative design and construction of engineered probiotics or microbial consortia. Especially, we highlight how genome-scale metabolic models can be applied to advance our understanding of the gut microbiota. Also, we review the recent applications of metabolic engineering in gut microbiome studies as well as discuss important challenges and opportunities.