Determination of phytoplankton community structure and biomass with HPLC-CHEMTAX and microscopic methods during winter and summer in the Qinzhou Bay of the Beibu Gulf

作者：Huizhu Pan, Mingben Xu, Caibi Lan, Jixian Ma, Jie Li, Jiachang Lu, Junxiang Lai · 发表于：Frontiers in Marine Science · 年份：2023 · DOI：10.3389/fmars.2023.1186106 · 被引用次数：10 · 研究领域：Marine and coastal ecosystems、Marine Biology and Ecology Research、Microbial Community Ecology and Physiology

The Qinzhou Bay, a typical semi-enclosed bay, is facing environmental pressure from local fast-growing industrial and aquacultural development. Dominant species of phytoplankton community (based on microscopic examination) show a trend of miniaturization, while pico-phytoplankton (based on CHEMTAX analysis) is widely distributed in Qinzhou Bay. However, most previous investigations of phytoplankton community based on microscopic method that undetected small-sized cell (< 3 μm), and limited by scarce studies on CHEMTAX analysis, the long-term dynamic data of small-size phytoplankton are lacking in Qinzhou Bay. It is recognized that combining microscopic examination with CHEMTAX analysis could provide a good taxonomic reliability for large cells and valuable information about small-size groups. In this study, microscopic examination and High Performance Liquid Chromatography (HPLC)-CHEMTAX analysis were employed to characterize the spatiotemporal variability of the phytoplankton community structure in Qinzhou Bay in winter and summer of 2021. The results of microscopic observations showed that the phytoplankton community was characterized by diatoms and dinoflagellates mainly. Diatoms dominated in both seasons, of which Skeletonema costatum bloom occurred in the summer. CHEMTAX analysis not only agreed well with microscopy data for diatoms and dinoflagellates, but also helped identification of other small-sized flagellates and cyanobacteria that hard to observe by micros...