Progesterone attenuates Th17-cell pathogenicity in autoimmune uveitis via Id2/Pim1 axis

作者：Xiuxing Liu, Chenyang Gu, Jianjie Lv, Qi Jiang, Wen Ding, Zhaohao Huang, Yidan Liu, Yuhan Su, Chun Zhang, Zhuping Xu, Xianggui Wang, Wenru Su · 发表于：Journal of Neuroinflammation · 年份：2023 · DOI：10.1186/s12974-023-02829-3 · 被引用次数：28 · 研究领域：Ocular Diseases and Behçet’s Syndrome、Cancer Mechanisms and Therapy、Vasculitis and related conditions

BACKGROUND: Autoimmune uveitis (AU) is the most common ophthalmic autoimmune disease (AD) and is characterized by a complex etiology, high morbidity, and high rate of blindness. AU remission has been observed in pregnant female patients. However, the effects of progesterone (PRG), a critical hormone for reproduction, on the treatment of AU and the regulatory mechanisms remain unclear. METHODS: To this end, we established experimental autoimmune uveitis (EAU) animal models and constructed a high-dimensional immune atlas of EAU-model mice undergoing PRG treatment to explore the underlying therapeutic mechanisms of PRG using single-cell RNA sequencing. RESULTS: We found that PRG ameliorated retinal lesions and inflammatory infiltration in EAU-model mice. Further single-cell analysis indicated that PRG reversed the EAU-induced expression of inflammatory genes (AP-1 family, S100a family, and Cxcr4) and pathological processes related to inflammatory cell migration, activation, and differentiation. Notably, PRG was found to regulate the Th17/Treg imbalance by increasing the reduced regulatory functional mediators of Tregs and diminishing the overactivation of pathological Th17 cells. Moreover, the Id2/Pim1 axis, IL-23/Th17/GM-CSF signaling, and enhanced Th17 pathogenicity during EAU were reversed by PRG treatment, resulting in the alleviation of EAU inflammation and treatment of AD. CONCLUSIONS: Our study provides a comprehensive single-cell map of the immunomodulatory effects of PR...