A Novel Fluorescent Probe for the Detection of Hydrogen Peroxide

作者：Kangkang Wang, Tingting Yao, Jia‐Yu Xue, Yan-qiu Guo, Xiaowei Xu · 发表于：Biosensors · 年份：2023 · DOI：10.3390/bios13060658 · 被引用次数：27 · 研究领域：Sulfur Compounds in Biology、Electrochemical sensors and biosensors、Molecular Sensors and Ion Detection

Hydrogen peroxide (H2O2) is one of the important reactive oxygen species (ROS), which is closely related to many pathological and physiological processes in living organisms. Excessive H2O2 can lead to cancer, diabetes, cardiovascular diseases, and other diseases, so it is necessary to detect H2O2 in living cells. Since this work designed a novel fluorescent probe to detect the concentration of H2O2, the H2O2 reaction group arylboric acid was attached to the fluorescein 3-Acetyl-7-hydroxycoumarin as a specific recognition group for the selective detection of hydrogen peroxide. The experimental results show that the probe can effectively detect H2O2 with high selectivity and measure cellular ROS levels. Therefore, this novel fluorescent probe provides a potential monitoring tool for a variety of diseases caused by H2O2 excess.