Isolation, Characterization, and Comparative Genomics of the Novel Potential Probiotics from Canine Feces

作者：Ngamlak Foongsawat, Sirinthorn Sunthornthummas, Kwannan Nantavisai, Komwit Surachat, Achariya Rangsiruji, Siriruk Sarawaneeyaruk, Kedvadee Insian, Sirapan Sukontasing, Nuttika Suwannasai, Onanong Pringsulaka · 发表于：Food Science of Animal Resources · 年份：2023 · DOI：10.5851/kosfa.2023.e28 · 被引用次数：12 · 研究领域：Probiotics and Fermented Foods、Microbial Metabolites in Food Biotechnology、Gut microbiota and health

Lactic acid bacteria (LAB) are commonly used as probiotics; however, not all LAB strains have the same beneficial effects. To successfully use LAB as probiotics in canines, LAB species should originate from the canine intestinal tract as they display host specificity. The objective of this study was to investigate the phenotypic and genomic traits of potential probiotic strains. LAB isolated from canine fecal samples. Twenty LAB samples were evaluated for their potential probiotic characteristics including resistance to low pH, bile salts, hydrophobicity, auto-aggregation, co-aggregation, adhesion to epithelia or mucosa, and production of inhibitory compounds. Additionally, we evaluated their safety and other beneficial effects to canine health, such as DPPH free radical scavenging, and β-galactosidase. Four strains demonstrated potential probiotic characteristics and were selected: Enterococcus hirae Pom4, Limosilactobacillus fermentum Pom5, Pediococcus pentosaceus Chi8, and Ligilactobacillus animalis FB2. Safety evaluations showed that all strains lacked hemolytic activity, could not produce biogenic amines, and did not carry any pathogenic genes. In addition, Lim. fermentum Pom5 and P. pentosaceus Chi8 displayed susceptibility to all antibiotics and concordant with the absence of antibiotic resistance genes. Based on their phenotypic and genomic characteristics, Lim. fermentum Pom5 and P. pentosaceus Chi8 were identified as potential probiotic candidates for dogs.