High endemicity of Opisthorchis viverrini infection among people in northern Cambodia confirmed by adult worm expulsion

作者：Bong-Kwang Jung, Sooji Hong, Taehee Chang, Jaeeun Cho, Seungwan Ryoo, Keon Hoon Lee, Jeong Gyu Lee, Woon‐Mok Sohn, Sung‐Jong Hong, Virak Khieu, Rekol Huy, Jong-Yil Chai · 发表于：Scientific Reports · 年份：2023 · DOI：10.1038/s41598-023-36544-z · 被引用次数：8 · 研究领域：Parasites and Host Interactions、Global Maternal and Child Health、Parasite Biology and Host Interactions

Opisthorchis viverrini infection is an emerging disease in Cambodia, especially in central and southeastern areas. However, its status in northern areas bordering Lao PDR has been relatively unknown. The present study was performed to investigate the status of O. viverrini infection among people in Preah Vihear and Stung Treng provinces through fecal examination to detect eggs and recovery of adult flukes from some of the egg-positive cases. Fecal examinations were performed on a total of 1101 people from 10 villages in the 2 provinces using the Kato-Katz thick smear technique. For recovery of adult flukes and other helminth parasites 10 volunteers positive for eggs of Opisthorchis viverrini and/or minute intestinal flukes (Ov/MIF), in Kampong Sangkae village, Preah Vihear province, were administered a single oral dose of 40 mg/kg praziquantel plus 5-10 mg/kg of pyrantel pamoate and purged with 40-50 g magnesium salts. Adult trematodes, together with nematodes and cestodes expelled in diarrheic stools were collected under a stereomicroscope or with the naked eye. The proportion of egg-positive cases for overall liver and intestinal helminths was high but not notably different between the 2 provinces, 65.5% in Preah Vihear versus 64.7% in Stung Treng. The average proportion of Ov/MIF egg-positive cases was 59.8%. A total of 315 adult specimens of O. viverrini were recovered from the 10 volunteers (4-98 specimens per individual; mean, 32). A smaller number of Haplorchis taichui...