Antimicrobial mechanisms and secondary metabolite profiles of Streptomyces hygroscopicus subsp. hygroscopicus 5–4 against banana fusarium wilt disease using metabolomics

作者：Tianyan Yun, Tao Jing, Xiaoping Zang, Dengbo Zhou, Kai Li, Yankun Zhao, Wei Wang, Jianghui Xie · 发表于：Frontiers in Microbiology · 年份：2023 · DOI：10.3389/fmicb.2023.1159534 · 被引用次数：18 · 研究领域：Plant-Microbe Interactions and Immunity、Genomics and Phylogenetic Studies、Plant Pathogens and Fungal Diseases

Fusarium wilt of bananas (FWB) is seriously affecting the sustainable development of the banana industry and is caused by the devastating soil-borne fungus Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4). Biological control is a promising strategy for controlling Fusarium wilt in bananas. We previously identified Streptomyces hygroscopicus subsp. hygroscopicus 5–4 with strong antifungal activity against the FWB. The most possible antimicrobial mechanism of strain 5–4 was explored using the metabolomics approach, light microscopy imaging, and transmission electron microscopy (TEM). The membrane integrity and ultrastructure of Foc TR4 was damaged after extract treatment, which was supported by the degradation of mycelium, soluble protein content, extracellular reducing sugar content, NADH oxidase activity, malondialdehyde content, mitochondrial membrane potential, and mitochondrial respiratory chain complex enzyme activity. The extracts of strain 5–4 cultivated at different times were characterized by a liquid chromatography–mass spectrometer (LC-MS). 647 known metabolites were detected in the extracts of strains 5–4. Hygromycin B, gluten exorphin B4, torvoside G, (z)-8-tetradecenal, piperitoside, sarmentosin, pubescenol, and other compounds were the main differential metabolites on fermentation culture for 7 days. Compared with strain 5–4 extracts, hygromycin B inhibited the mycelial growth of Foc TR4, and the EC 50 concentration was 7.4 μg/mL. These results showed...