Punicalagin prevents obesity-related cardiac dysfunction through promoting DNA demethylation in mice

作者：Shengjie Pei, Run Liu, Qingqing Ma, Peng Jiang, Xin He, Zhongshi Qi, Jiacheng Fang, Yang Xu, Zirui Yao, Xiaoqian Liu, Xianfeng Jing, Lei Chen, Duo Li · 发表于：Food Science and Human Wellness · 年份：2023 · DOI：10.26599/fshw.2022.9250123 · 被引用次数：5 · 研究领域：Pomegranate: compositions and health benefits、Bioactive Compounds in Plants、Cynara cardunculus studies

The aim of this study was to investigate whether punicalagin (PU) could prevent obesity-related cardiac dysfunction by promoting DNA demethylation, and to explore its possible mechanism. C57BL/6J mice were fed with standard diet, high-fat diet (HFD), HFD supplemented with resveratrol, low-dose PU (LPU) and high-dose PU (HPU) for eight weeks. Compared with HFD group, body weight was significantly lower in PU treatment groups, number of cardiomyocytes and the protein level of myosin heavy chain 7B were significantly higher in PU treatment groups. Levels of 5-hydroxymethylcytosine and 5-formylcytosine were significantly lower in HFD group than other groups. Compared with the HFD group, the protein level of ten-eleven translocation enzyme (TET) 2 was significantly higher in PU treatment groups, p-AMP-activated protein kinase (AMPK)/AMPK was significantly higher in LPU group. Levels of total antioxidant capacity and the protein levels of complexes II/III/V, oxoglutarate dehydrogenase, succinate dehydrogenase B and fumarate hydrolase were significantly lower in HFD group than PU treatment groups. The ratio of (succinic acid + fumaric acid)/ α -ketoglutarate was significantly higher in HFD group than other groups. In conclusion, PU up-regulated TETs enzyme activities and TET2 protein stability through alleviating mitochondrial dysfunction and activating AMPK, so as to promote DNA demethylation, thus preventing obesity-related&nb...