3D bioprinted hydrogel/polymer scaffold with factor delivery and mechanical support for growth plate injury repair

作者：Minjie Fan, Lei Qiang, Yiwei Wang, Yihao Liu, Hanjie Zhuang, Ruoyi Guo, Yulong Ben, Qiang Li, Pengfei Zheng · 发表于：Frontiers in Bioengineering and Biotechnology · 年份：2023 · DOI：10.3389/fbioe.2023.1210786 · 被引用次数：21 · 研究领域：3D Printing in Biomedical Research、Mesenchymal stem cell research、Osteoarthritis Treatment and Mechanisms

Introduction: Growth plate injury is a significant challenge in clinical practice, as it could severely affect the limb development of children, leading to limb deformity. Tissue engineering and 3D bioprinting technology have great potential in the repair and regeneration of injured growth plate, but there are still challenges associated with achieving successful repair outcomes. Methods: In this study, GelMA hydrogel containing PLGA microspheres loaded with chondrogenic factor PTH(1–34) was combined with BMSCs and Polycaprolactone (PCL) to develop the PTH(1–34)@PLGA/BMSCs/GelMA-PCL scaffold using bio-3D printing technology. Results: The scaffold exhibited a three-dimensional interconnected porous network structure, good mechanical properties, biocompatibility, and was suitable for cellchondrogenic differentiation. And a rabbit model of growth plate injury was appliedto validate the effect of scaffold on the repair of injured growth plate. The resultsshowed that the scaffold was more effective than injectable hydrogel in promotingcartilage regeneration and reducing bone bridge formation. Moreover, the addition ofPCL to the scaffold provided good mechanical support, significantly reducing limbdeformities after growth plate injury compared with directly injected hydrogel. Discussion: Accordingly, our study demonstrates the feasibility of using 3D printed scaffolds for treating growth plate injuries and could offer a new strategy for the development of growth plate tissue engine...