Role of CST1 in Promoting Gastric Cancer Metastasis and Analysis of Its Mechanism

作者：Jianfei Wu, Xinmei Hu, Xinyu Peng, Xiu Zhang, Jianjun Zhang, Peng Liu · 发表于：Proceedings of Anticancer Research · 年份：2023 · DOI：10.26689/par.v7i3.4861 · 研究领域：Cancer Research and Treatments、Peptidase Inhibition and Analysis、Cancer-related gene regulation

Objective: To elucidate the role and mechanism of cysteine protease inhibitor SN (CST1) in promoting the metastasis of gastric cancer. Methods: (1) Firstly, 6 pairs of cancer and paracancer tissue samples from gastric cancer patients without distant metastasis and 5 pairs of cancer and paracancer tissue samples from gastric cancer patients with peritoneal metastasis were collected for transcriptome sequencing. Statistical analysis was performed on the sequencing results to identify significantly upregulated differential genes. (2) Another 75 pairs of cancer and paracancer tissue samples were collected from gastric cancer patients, and the protein and total RNA of gastric cancer tissue samples were extracted. Western blot and quantitative reverse transcription polymerase chain reaction (qRT-PCR) were used to detect the expression of CST1 protein and messenger RNA (mRNA) in gastric cancer and adjacent tissues. (3) The total protein and total RNA of AGS, BGC823, HGC-27, MGC803, MKN45, SGC7901, and SNU-1 gastric cancer cells and normal gastric mucosal epithelial cells GES-1 were extracted. Western blot and qRT-PCR were used to detect CST1 protein and mRNA expression. Results: (1) The significantly upregulated differential gene CST1 was screened, and the expression of CST1 in gastric cancer tissues was significantly higher than that in adjacent tissues. (2) Compared with normal gastric mucosal epithelial cells GES-1, the expression of CST1 in gastric cancer cell lines was upregula...