Virulence-related factors and antimicrobial resistance in Proteus mirabilis isolated from domestic and stray dogs

作者：Lijuan Liu, Zhiyou Dong, Shengquan Ai, Shanyu Chen, Mengyao Dong, Qianlan Li, Ziyao Zhou, Haifeng Liu, Zhijun Zhong, Xiaoping Ma, Yanchun Hu, Zhihua Ren, Hualin Fu, Gang Shu, Xianmeng Qiu, Guangneng Peng · 发表于：Frontiers in Microbiology · 年份：2023 · DOI：10.3389/fmicb.2023.1141418 · 被引用次数：28 · 研究领域：Antibiotic Resistance in Bacteria、Pharmaceutical and Antibiotic Environmental Impacts、Bacterial biofilms and quorum sensing

Introduction Proteus mirabilis is a multi-host pathogen that causes diseases of varying severity in a wide range of mammals, including humans. Proteus mirabilis is resistant to multiple antibiotics and has acquired the ability to produce expanded spectrum of β-lactamases, leading to serious public health problems. However, the available information on P. mirabilis isolated from feces of dogs, is still poorly understood, as is the correlation between its virulence-associated genes (VAGs) and antibiotic resistance genes (ARGs). Method In this study, we isolated 75 strains of P. mirabilis from 241 samples, and investigated the swarming motility, biofilm formation, antimicrobial resistance (AMR), distribution of VAGs and ARGs, as well as the presence of class 1, 2, and 3 integrons in these isolates. Results Our findings suggest a high prevalence of intensive swarming motility and strong biofilm formation ability among P. mirabilis isolates. Isolates were primarily resistant to cefazolin (70.67%) and imipenem (70.67%). These isolates were found to carry ureC , FliL , ireA , zapA , ptA , hpmA , hpmB , pmfA , rsbA , mrpA , and ucaA with varying prevalence levels of 100.00, 100.00, 100.00, 98.67, 98.67, 90.67, 90.67, 90.67, 90.67, 89.33, and 70.67%, respectively. Additionally, the isolates were found to carry aac(6′)-Ib , qnrD , floR , bla CTX-M , bla CTX-M-2 , bla OXA-1 , bla TEM , tetA , tetB and tetM with varying prevalence levels of 38.67, 32.00, 25.33, 17.33, 16.00, 10.67, 5.33,...