Long-term cold, freezing and drought: overlapping and specific regulatory mechanisms and signal transduction in tea plant (Camellia sinensis (L.) Kuntze)

作者：Lidiia Samarina, Songbo Wang, L. S. Malyukova, Aleksandr Bobrovskikh, Alexey V. Doroshkov, N. G. Koninskaya, Ruset M. Shkhalakhova, A. O. Matskiv, Jaroslava Fedorina, Anastasia Fizikova, Karina Manakhova, Svetlana Loshkaryova, Tsiala Tutberidze, Alexey Ryndin, Е. К. Хлесткина · 发表于：Frontiers in Plant Science · 年份：2023 · DOI：10.3389/fpls.2023.1145793 · 被引用次数：18 · 研究领域：Plant Gene Expression Analysis、Photosynthetic Processes and Mechanisms、Plant Molecular Biology Research

Introduction Low temperatures and drought are two main environmental constraints reducing the yield and geographical distribution of horticultural crops worldwide. Understanding the genetic crosstalk between stress responses has potential importance for crop improvement. Methods In this study, Illumina RNA-seq and Pac-Bio genome resequencing were used to annotate genes and analyze transcriptome dynamics in tea plants under long-term cold, freezing, and drought. Results The highest number of differentially expressed genes (DEGs) was identified under long-term cold (7,896) and freezing (7,915), with 3,532 and 3,780 upregulated genes, respectively. The lowest number of DEGs was observed under 3-day drought (47) and 9-day drought (220), with five and 112 genes upregulated, respectively. The recovery after the cold had 6.5 times greater DEG numbers as compared to the drought recovery. Only 17.9% of cold-induced genes were upregulated by drought. In total, 1,492 transcription factor genes related to 57 families were identified. However, only 20 transcription factor genes were commonly upregulated by cold, freezing, and drought. Among the 232 common upregulated DEGs, most were related to signal transduction, cell wall remodeling, and lipid metabolism. Co-expression analysis and network reconstruction showed 19 genes with the highest co-expression connectivity: seven genes are related to cell wall remodeling ( GATL7 , UXS4 , PRP-F1 , 4CL , UEL-1 , UDP-Arap , and TBL32 ), four genes a...