A high molecular weight silk fibroin scaffold that resists degradation and promotes cell proliferation

作者：Mengmeng Wang, Ying Wang, Peng Pan, Xueping Liu, Wenjing Zhang, Cheng Hu, Mingzhong Li · 发表于：Biopolymers · 年份：2023 · DOI：10.1002/bip.23554 · 被引用次数：16 · 研究领域：Silk-based biomaterials and applications、Electrospun Nanofibers in Biomedical Applications、Biochemical and Structural Characterization

Abstract The regulation of the biodegradation rate of 3D‐regenerated silk fibroin scaffolds and the avoidance of premature collapse are important concerns for their effective applications in tissue engineering. In this study, bromelain, which is specific to sericin, was used to remove sericin from silk, and high molecular weight silk fibroin was obtained after the fibroin fibers were dissolved. Afterwards, a 3D scaffold was prepared via freeze‐drying. The Sodium dodecyl sulfate–polyacrylamide gel electrophoresis results showed that the average molecular weight of the regenerated silk fibroin prepared by using the bromelain‐degumming method was approximately 142.2 kDa, which was significantly higher than that of the control groups prepared by using the urea‐ and Na 2 CO 3 ‐degumming methods. The results of enzyme degradation in vitro showed that the biodegradation rate and internal three‐dimensional structure collapse of the bromelain‐degumming fibroin scaffolds were significantly slower than those of the two control scaffolds. The proliferation activity of human umbilical vein vascular endothelial cells inoculated in bromelain‐degumming fibroin scaffolds was significantly higher than that of the control scaffolds. This study provides a novel preparation method for 3D‐regenerated silk fibroin scaffolds that can effectively resist biodegradation, continuously guide cell growth, have good biocompatibility, and have the potential to be used for the regeneration of various connect...