Microbial landscapes of the rhizosphere soils and roots of Luffa cylindrica plant associated with Meloidogyne incognita

作者：Ling Qu, Kang Dai, Jie Wang, Li Cao, Zhongchen Rao, Richou Han · 发表于：Frontiers in Microbiology · 年份：2023 · DOI：10.3389/fmicb.2023.1168179 · 被引用次数：12 · 研究领域：Nematode management and characterization studies、Plant-Microbe Interactions and Immunity、Plant Pathogens and Fungal Diseases

Introduction The root-knot nematodes (RKN), especially Meloidogyne spp., are globally emerging harmful animals for many agricultural crops. Methods To explore microbial agents for biological control of these nematodes, the microbial communities of the rhizosphere soils and roots of sponge gourd ( Luffa cylindrica ) infected and non-infected by M. incognita nematodes, were investigated using culture-dependent and -independent methods. Results Thirty-two culturable bacterial and eight fungal species, along with 10,561 bacterial and 2,427 fungal operational taxonomic units (OTUs), were identified. Nine culturable bacterial species, 955 bacterial and 701 fungal OTUs were shared in both four groups. More culturable bacterial and fungal isolates were detected from the uninfected soils and roots than from the infected soils and roots (except no fungi detected from the uninfected roots), and among all samples, nine bacterial species ( Arthrobacter sp., Bacillus sp., Burkholderia ambifaria , Enterobacteriaceae sp., Fictibacillus barbaricus , Microbacterium sp., Micrococcaceae sp., Rhizobiaceae sp., and Serratia sp.) were shared, with Arthrobacter sp. and Bacillus sp. being dominant. Pseudomonas nitroreducens was exclusively present in the infested soils, while Mammaliicoccus sciuri , Microbacterium azadirachtae , and Priestia sp., together with Mucor irregularis , Penicillium sp., P. commune , and Sordariomycetes sp. were found only in the uninfected soils. Cupriavidus metallidurans ,...