The first high-quality chromosome-level genome of the Sipuncula Sipunculus nudus using HiFi and Hi-C data

作者：Zheng Zhe, Zhuoxin Lai, Bin Wu, Xinlin Song, Wei Zhao, Ruzhuo Zhong, Jiawei Zhang, Yongshan Liao, Chuangye Yang, Yuewen Deng, Junpu Mei, Zhen Yue, Jianbo Jian, Qingheng Wang · 发表于：Scientific Data · 年份：2023 · DOI：10.1038/s41597-023-02235-7 · 被引用次数：11 · 研究领域：Genomics and Phylogenetic Studies、Genetic diversity and population structure、Parasite Biology and Host Interactions

Sipuncula is a class of exocoelomic unsegmented animals whose evolutionary relationships are unresolved. The peanut worm Sipunculus nudus is a globally distributed, economically important species belonging to the class Sipuncula. Herein, we present the first high-quality chromosome-level assembly of S. nudus based on HiFi reads and high-resolution chromosome conformation capture (Hi-C) data. The assembled genome was 1,427 Mb, with a contig N50 length of 29.46 Mb and scaffold N50 length of 80.87 Mb. Approximately 97.91% of the genome sequence was anchored to 17 chromosomes. A BUSCO assessment showed that 97.7% of the expectedly conserved genes were present in the genome assembly. The genome was composed of 47.91% repetitive sequences, and 28,749 protein-coding genes were predicted. A phylogenetic tree demonstrated that Sipuncula belongs to Annelida and diverged from the common ancestor of Polychaeta. The high-quality chromosome-level genome of S. nudus will serve as a valuable reference for studies of the genetic diversity and evolution of Lophotrochozoa.